Development and validation of a prediction model for fat mass in children and adolescents: meta-analysis using individual participant dataBMJ 2019; 366 doi: https://doi.org/10.1136/bmj.l4293 (Published 24 July 2019) Cite this as: BMJ 2019;366:l4293
- Mohammed T Hudda, PhD student1,
- Mary S Fewtrell, professor of paediatric nutrition2,
- Dalia Haroun, associate professor in nutrition3,
- Sooky Lum, honorary senior research associate4,
- Jane E Williams, research nurse2,
- Jonathan C K Wells, professor of anthropology and paediatric nutrition2,
- Richard D Riley, professor of biostatistics5,
- Christopher G Owen, professor of epidemiology1,
- Derek G Cook, professor of epidemiology1,
- Alicja R Rudnicka, professor of statistical epidemiology1,
- Peter H Whincup, professor of cardiovascular epidemiology1,
- Claire M Nightingale, lecturer in medical statistics and epidemiology1
- 1Population Health Research Institute, St George’s, University of London, London SW17 0RE, UK
- 2Population, Policy and Practice Programme, UCL Great Ormond Street Institute of Child Health, London, UK
- 3College of Natural and Health Sciences, Department of Public Health and Nutrition, Zayed University, Dubai, UAE
- 4Respiratory, Critical Care and Anaesthesia section of III Programme, UCL Great Ormond Street Institute of Child Health, London, UK
- 5Centre for Prognosis Research, Research Institute for Primary Care and Health Sciences, Keele University, Staffordshire, UK
- Correspondence to: C M Nightingale @mohammedhudda on Twitter) (or
- Accepted 6 June 2019
Objectives To develop and validate a prediction model for fat mass in children aged 4-15 years using routinely available risk factors of height, weight, and demographic information without the need for more complex forms of assessment.
Design Individual participant data meta-analysis.
Setting Four population based cross sectional studies and a fifth study for external validation, United Kingdom.
Participants A pooled derivation dataset (four studies) of 2375 children and an external validation dataset of 176 children with complete data on anthropometric measurements and deuterium dilution assessments of fat mass.
Main outcome measure Multivariable linear regression analysis, using backwards selection for inclusion of predictor variables and allowing non-linear relations, was used to develop a prediction model for fat-free mass (and subsequently fat mass by subtracting resulting estimates from weight) based on the four studies. Internal validation and then internal-external cross validation were used to examine overfitting and generalisability of the model’s predictive performance within the four development studies; external validation followed using the fifth dataset.
Results Model derivation was based on a multi-ethnic population of 2375 children (47.8% boys, n=1136) aged 4-15 years. The final model containing predictor variables of height, weight, age, sex, and ethnicity had extremely high predictive ability (optimism adjusted R2: 94.8%, 95% confidence interval 94.4% to 95.2%) with excellent calibration of observed and predicted values. The internal validation showed minimal overfitting and good model generalisability, with excellent calibration and predictive performance. External validation in 176 children aged 11-12 years showed promising generalisability of the model (R2: 90.0%, 95% confidence interval 87.2% to 92.8%) with good calibration of observed and predicted fat mass (slope: 1.02, 95% confidence interval 0.97 to 1.07). The mean difference between observed and predicted fat mass was −1.29 kg (95% confidence interval −1.62 to −0.96 kg).
Conclusion The developed model accurately predicted levels of fat mass in children aged 4-15 years. The prediction model is based on simple anthropometric measures without the need for more complex forms of assessment and could improve the accuracy of assessments for body fatness in children (compared with those provided by body mass index) for effective surveillance, prevention, and management of clinical and public health obesity.
With the increasing prevalence of obesity in children globally,1 such as in the United Kingdom, where about one third of children aged 2-15 years are overweight or obese,2 high body fatness in childhood represents a serious public health problem. High levels of body fatness in childhood have been associated with both overweight and obesity and increased risks of non-communicable diseases in adulthood—notably type 2 diabetes and cardiovascular diseases.34567
Accurate and practical methods for quantifying body fatness in children are essential for effective monitoring, prevention, and management of high body fatness, overweight, and obesity in childhood.89 Body mass index (BMI), the most widely used marker of childhood body fatness in clinical and public health practice, has serious limitations as a marker of body fatness in children.91011 Firstly, as a weight based measure, it does not discriminate between lean (fat-free mass) and fat mass, which can vary substantially in those with a given BMI.10 Secondly, height squared provides poor height standardisation of weight in children—a higher power is needed to obtain height standardisation.121314 Finally, BMI in childhood is not a consistent marker of body fatness across different ethnic groups. In the UK and the United States, BMI has been shown to overestimate body fatness in black African children and underestimate body fatness in children of Asian origin.1516171819 Similar problems have been reported in other settings; BMI under estimates body fatness in South Asian girls and over estimates body fatness in Pacific Island girls in New Zealand.20
Although imaging (by dual energy x ray absorptiometry or magnetic resonance imaging), densitometric, and isotope dilution methods are available and accurate, they are unsuitable for routine clinical or public health assessment of body fatness.1121 Simple methods for body fatness assessment, based on routinely available measurements (particularly weight and height) and valid in a range of populations would be of considerable value.
We examined whether weight and height as opposed to BMI could provide more accurate assessments of fat mass, particularly using prediction methods that have shown promise in estimating disease risks.222324
We report on the development and validation of a prediction model to estimate fat mass accurately in UK children aged 4-15 years of different ethnic origins, based on weight, height, and routinely available basic demographic information.
Data sources and study population
For this investigation we pooled data from four cross sectional studies for the development of a prediction model, with a fifth study (not available at the time of model derivation) for external validation. All studies included data on weight, height, and reference standard body fatness assessments based on the deuterium dilution method.
Data from four separate cross sectional studies17252627 (supplementary table 1), identified as the four available UK population based studies, which contained deuterium dilution measurements together with weight and height measurements in more than 200 children aged 4-15 years, were obtained and pooled for analysis (n=2375). Each of these studies used a similar protocol when conducting the deuterium dilution method to measure total body water (and indirectly fat mass), as described elsewhere.15 Three of the four studies included multi-ethnic populations; assessment of ethnicity was based on a combination of self reported parental information on parental ethnicity17 and child ethnicity,172627 with self reported participant information on ethnicity for older children.2526 Ethnic group categories were based on the 2001 UK census (supplementary table 1).
External validation data
Data from a smaller separate UK cross sectional study at the 11 year follow-up visit within the Avon Longitudinal Study of Parents and Children (ALSPAC)28 were obtained for external validation. ALSPAC is a birth cohort study containing detailed assessments from predominantly white children born in the Bristol area between April 1991 and December 1992, including information on height, weight, sex, ethnicity, and age. At the 11 year follow-up visit, a subsample of the cohort (stratified by sex and BMI to represent the whole cohort) was recruited to participate in a further study that involved assessment of fat mass using the deuterium dilution method alongside measures of height and weight taken simultaneously.29 Ethnicity was based on a combination of self reported parental information on parental ethnicity.
Defining the outcome of prediction models
Our primary aim was to develop a model for predicting fat mass in childhood, which could be estimated directly or indirectly (by predicting fat-free mass from models and then subtracting resulting estimates from known weight) based on deuterium dilution measurements. Firstly, we investigated the potential for modelling fat mass directly or indirectly by examining the distributions of fat mass and fat-free mass in relation to height (one of the strongest predictors of body composition) in boys and girls separately. This showed that a regression model for fat-free mass better met the assumptions of linear regression (more details in Appendix 1). The distribution of fat-free mass (both in boys and girls separately and combined) was positively skewed (supplementary figure 1) and showed increased heterogeneity with increases in height and weight. Fat-free mass, transformed using natural logarithms, was therefore the outcome in the main analyses.
In the model development stage, we considered weight, height, age, sex, and ethnic group as candidate predictors (variables). Our derivation data, once restricted to those with fat-free mass or fat mass assessment, had no missing data on any of the candidate predictors. The sample size of 2375 participants meant that the number of candidate predictors being considered (along with non-linear terms) far exceeded both the minimum 10 people per candidate predictor rule of thumb30 and the minimum sample size requirements for prediction models proposed elsewhere.31 Ethnicity was based on self reported parental information on parental ethnicity. For the present analyses, we categorised child ethnicity as white (European origin), black (black children of African and Caribbean descent), South Asian (children of Indian, Pakistani, Bangladeshi, and Sri Lankan descent), other Asian (predominantly East Asian origins), and other (predominantly mixed ethnicity) groups.
Statistical analysis for model development
Stata v14 was used for all analyses. We followed the TRIPOD (transparent reporting of a multivariable model for individual prognosis or diagnosis) guidance for development and reporting of multivariable prediction models.32 To avoid data splitting we used all four available studies for model development.33 A linear regression was used with the natural logarithm of fat-free mass as the outcome, and weight, height, age, sex, and ethnic group as candidate predictors (variables). Using a stepwise approach through backwards elimination, beginning with a model that included all predictors, we excluded candidate predictors from the saturated model based on their statistical significance (Wald test P>0.05). Non-linear relations between outcome and continuous predictors were considered by identifying, at each iterative step of the stepwise process, the best fitting fractional polynomial terms3435 (using Stata command mfp36). This model development process led to a final model for the prediction of natural logarithm of fat-free mass (and subsequently for fat mass=weight−exp(prediction of natural logarithm of fat-free mass)) based on the selected predictors along with their corresponding estimated β coefficients and the associated intercept term. Although heterogeneity and clustering of patients across or within studies was not considered for model development, we checked the impact of this using an internal-external validation approach.
Model performance and internal validation
The performance of the final model was assessed using several approaches:
• R2—proportion of the variance in natural logarithm of fat-free mass explained by the included predictors
• Root mean square error (RMSE)—the average difference between the predicted and observed values. The RMSE of fat mass predictions was also assessed overall and within subgroups for age, ethnicity, and sex
• Calibration slope—based on model regressing observed on predicted values of natural logarithm of fat-free mass (with a slope of 1 being ideal)
• Calibration-in-the-large—intercept term from the model regressing observed on predicted values of natural logarithm of fat-free mass (with an intercept of 0 being ideal)
• Comparing mean observed with mean predicted values of natural logarithm of fat-free mass.
Calibration was also assessed graphically by displaying fat-free mass and fat mass on a calibration plot with a local regression (loess) smoother fitted across all children
We carried out internal validation to estimate optimism (the level of model overfitting)32 and correct measures of predictive performance (R2, calibration slope, and calibration-in-the-large) for model overfitting by bootstrapping32 1000 samples of the derivation data (with replacement). The entire variable selection process, including the choosing of the fractional polynomial terms, was repeated within the model development for each of the 1000 bootstrap samples. This led to a set of 1000 bootstrap models that were derived using the same methods as in our original model development. We then applied each of these bootstrap sample models within the original dataset to estimate optimism in the performance statistics (difference in test performance and apparent bootstrap performance) of R2, calibration slope, and calibration-in-the-large (see Appendix 2 for further details), referred to as adjusted R2, adjusted calibration slope, and adjusted calibration-in-the-large, respectively. To adjust for optimism after model development, we obtained estimates of a uniform shrinkage factor (the average calibration slope from each of the bootstrap samples) and multiplied these by the original β coefficients to obtain optimism adjusted coefficients.3237 At this stage, we re-estimated the intercept of the model based on the adjusted coefficients to maintain overall model calibration,32 producing a final model.
It is important to examine the generalisability of a prediction model developed using the process discussed. Owing to the limited availability of appropriate external datasets, we conducted internal-external validation3839 to further assess the performance of the derived model. This internal-external approach3839 involved cross validation, omitting one of each of the four studies in turn from the development dataset, and developing a model within the remaining three datasets. The following three steps were undertaken: (1) using the same model development strategy, we developed a model on three of the four studies and obtained the β coefficients from the model predicting natural logarithm of fat-free mass; (2) the predictive performance of the model from the first step was then assessed (overall and within sex and ethnic groups) within the fourth external validation study data in terms of accuracy of predicted fat mass (the primary outcome) by means of the calibration slope, calibration-in-the-large, and the R2 measures; and (3) we repeated the first two steps until we had assessed external validation for each of the four studies.
We assessed overfitting in each round of the cross validation and obtained a uniform shrinkage factor,37 which was applied to the β coefficients from step 1. Calibration slope, calibration-in-the-large, and R2 measures derived from this procedure for each of the studies were then pooled and estimated via a random effects meta-analysis to assess the heterogeneity across studies (with the τ2 statistic estimated using the Mantel-Haenszel method). The variance of R2 was estimated using the Wald type method outlined previously40 and used to pool the values.
We applied our final prediction model to each participant in the external validation dataset based on his or her respective predictor values. In a small number of children with missing ethnicity, we reclassified missing ethnicity data as white to produce an estimate of fat mass. The performance of the model for predicting fat mass, by sex and overall, was assessed using the calibration slope, calibration-in-the-large, R2, and RMSE and by comparing mean observed values with mean predicted values. We also assessed the overall calibration of the model graphically in terms of fat mass by plotting agreement between predicted and observed values across 10ths of predicted values. Finally, we re-estimated the intercept term from the final model for the external data to maintain the calibration of the model and reassessed the performance statistics.
Patient and public involvement
No patients were involved in setting the research question or the outcome measures, nor were they involved in the study design or implementation. No patients were involved in the interpretation or writing up of results. There are no plans to disseminate the results of the research to study participants or the relevant patient community.
The pooled derivation dataset (four studies) included 2375 children, predominantly of white (37.3%, n=885), black (23.3%, n=553), and South Asian (24.7%, n=586) ethnicity, aged 4.0-15.9 years (median age 9.6 years, 47.8% (n=1136) boys) with complete information on anthropometric, demographic, and body fatness measurements (table 1). The external validation dataset included 176 children predominantly of white ethnicity and aged 11-12 years (47.7% boys, n=84), with complete data on anthropometric and body fatness measurements and missing data on ethnicity in a small number of children (<10%). For the pooled derivation dataset, the distribution of age within each of the four individual studies varied—one study contained children across the full age range (albeit children of only white ethnic origin), whereas the other three studies each contained a restricted age range, but with noticeable ethnic diversity.
Model development and apparent performance
The final multivariable model included all five candidate predictors of height, weight, age, sex, and ethnic group (ie, none were excluded). Fractional polynomial terms for the continuous predictors (height, weight, and age) were included in the final model to allow for non-linear relations (table 2). The model showed excellent apparent predictive performance for natural logarithm of fat-free mass (table 3; R2=94.8%, RMSE=0.068) and was perfectly calibrated in the development data (apparent slope=1, apparent calibration-in-the-large=0). This is confirmed by the calibration plot, assessing agreement between observed and predicted fat-free mass and fat mass (fig 1). The difference between the mean observed and mean predicted values of natural logarithm of fat-free mass was zero. The RMSE values for fat mass were 2.0 kg in girls and 1.9 kg in boys and ranged between 0.9 kg and 3.3 kg within the one year age groups. Within ethnic groups, the RMSE ranged between 1.7 kg among South Asian children and 2.4 kg among black children.
Bootstrap internal validation showed little model overfitting, which was reflected in the similar apparent and optimism adjusted performance statistics (table 3). After we had adjusted for overfitting, the final prediction model maintained a high proportion of the variance in natural logarithm of fat-free mass with an adjusted R2 value of 94.8%. The bootstrapping approach provided a shrinkage factor of practically 1 (ie, there was no important overfitting, with the mean calibration slope equal to 1 from the bootstrap models when tested in the original data). We also calculated the uniform shrinkage factors suggested previously,37 and this gave a value of 0.99858, again close to 1. We chose to use this method because it was slightly smaller than the bootstrap value, which was applied to the original β coefficients from the model to obtain optimism adjusted coefficients before re-estimation of the intercept term. Box 1 shows the prediction equation for the estimation of fat mass in children aged 4-15 years, with examples of how to calculate fat mass using the equation.
Box 1 Final equation for prediction of fat mass in children aged 4-15 yearsFat mass=weight−exp[0.3073×height2−10.0155×weight−1+0.004571×weight+0.01408×BA−0.06509×SA−0.02624×AO−0.01745×other−0.9180×ln(age)+0.6488×age0.5+0.04723×male+2.8055]
exp=exponential function, ln=natural logarithmic transformation
Score 1 if child is of black (BA), south Asian (SA), other Asian (AO), or other (other) ethnic origins and score 0 if not
If child is of unknown ethnic group, treat as of white ethnic origins
Height is measured in metres, weight in kilograms, age in years, and fat mass in kilograms
For a 6 year old white boy of height 1.4 m and weight 37 kg, fat mass would be estimated as:
For a 12 year old black girl with a height of 1.6 m and a weight of 42 kg, fat mass would be estimated as:
Using the cross validation approach, we developed a model in each of the three studies and applied this within the fourth study. Assessments of model overfitting showed low levels of optimism at each round of cross validation (shrinkage factor=0.998 for each round). Within each of the studies being used as a validation dataset, after adjusting for optimism, the calibration slopes were close to 1 and the calibration-in-the-large values were close to 0, suggesting excellent model calibration in each of these four study populations (fig 2, supplementary table 2).
The pooled calibration slopes and calibration-in-the-large values across the four studies for fat mass were 1.00 (95% confidence interval 0.95 to 1.04) and −0.29 (−0.83 to 0.25), respectively, suggesting that, on average across the four populations, the model is likely to calibrate well. The pooled R2 value for fat mass was 89.7% (95% confidence interval 87.8% to 91.7%), which indicates that the model, on average, explains a high proportion of the variance in fat mass. The τ2 values for the calibration slope, calibration-in-the-large, and R2 measures were 0.002, 0.267, and 0.0004, respectively, suggesting little heterogeneity across the four populations. The calibration slopes and calibration-in-the-large values within sex and ethnic groups showed good calibration for all subgroups during each round of cross validation, suggesting that the final model is likely to calibrate well for children of both sexes and each ethnic group (supplementary figures 2 and 3).
We applied our final prediction model (box 1) to the independent population of children aged 11-12 years, reclassifying the small number of children with missing information on ethnicity as being from the white reference group. The resulting R2 value from the model was 90.0% (95% confidence interval 87.2% to 92.8%), with a moderate RMSE of 2.6 kg, and the model had average calibration in terms of fat mass (fig 3); with a slope of 1.02 (95% confidence interval 0.97 to 1.07) and calibration-in-the-large of −1.58 kg (95% confidence interval −2.29 to −0.86 kg) (table 4). The mean difference between observed and predicted fat mass was −1.29 kg (95% confidence interval −1.62 to −0.96 kg). The final model was observed to perform better in girls than in boys (table 4). After recalibration of the intercept, the R2 value from the model was 90.0% (95% confidence interval 87.1% to 92.8%), with a RMSE of 2.4 kg, and the model had a calibration slope of 1.06 (95% confidence interval 1.01 to 1.11) and calibration-in-the-large of 0.21 kg (95% confidence interval −0.42 to 0.85 kg).
In our final model, we tested and found two-way interactions between sex and weight and sex and age (along with their appropriate non-linear fractional polynomial terms) to be statistically significant at the 5% level. However, inclusion of additional terms for sex×weight and sex×age did not improve the apparent performance of the model (R2=94.9%, RMSE=0.068), with little difference between the Akaike’s Information Criterion (compares the relative quality of a set of statistical models for a given dataset) from models including and excluding these terms. Therefore, these interaction terms were not added to the previously described prediction model.
We also used two approaches to investigate the use of the proposed model to estimate fat mass in childhood when ethnic origins were unknown—omitting ethnic group as a predictor from the model, and treating children of unknown ethnic origin as being white (reference group) for fat mass predictions. Both approaches were carried out and compared using an internal-external validation approach. Fat mass predictions from both approaches had similar levels of bias when compared with observed fat mass values, suggesting that children of unknown ethnic origins can be treated as white with little effect on the predictive performance.
Finally, to investigate the direct approach of predicting fat mass, we repeated the model development strategy using the natural logarithm of fat-free mass as the primary outcome. The apparent performance of this model (R2=83.4%) was much less favourable than the performances of the main analyses using the natural logarithm of fat-free mass as the primary outcome.
We developed a new prediction equation, based on readily available measures of height, weight, age, sex, and ethnic group, to estimate fat mass levels (kg) for children aged 4-15 years using a large representative sample from the UK. We then validated the model both internally and externally—firstly using a cross validation approach within the derivation population and then within an independent dataset of children aged 11-12 years. Both overall and within age, sex, and ethnic subgroups, the developed model showed high predictive ability, with excellent calibration; low individual error, with root mean square error (RMSE) values less than 3.3 kg; and useful R2 values greater than 88% from the derivation, cross validation, and external validation datasets. The average individual error associated with the predictions in the independent dataset was low, with a RMSE of 2.6 kg.
Comparison with other studies
To our knowledge, few previous studies have developed and validated prediction models to estimate fat mass in children and adolescents based solely on weight, height, and demographic factors.41 Most previously derived models for this purpose have focused on older children and adolescents from the United States with body fatness assessed using dual energy x ray absorptiometry.414243444546 Moreover, modelling has predominantly been based on the prediction of percentages and not absolute values of body fatness, making it difficult to compare the predictive ability of models. The developed models, which have been shown to estimate the percentage of body fatness to a high level, with R2 values greater than 82%, have relied on additional measurements, including skinfold thickness, waist circumference, or bioelectrical impedance to estimate body fatness.4243444546 However, a previously developed model in 12-20 year-olds in the US included the same predictors as in our final model, of height and weight (in the form of a fractional polynomial non-linear term of body mass index, BMI) as well as sex, age, and ethnicity to estimate the percentage of body fatness.41 That model performed well, explaining a high proportion of the variance in body fatness percentage (R2=79.4%). The RMSE was not presented, however, making direct comparisons of accuracy between the models difficult.
Strengths and limitations of this study
This study has several strengths. The derivation dataset was sufficiently large, with complete information on candidate predictors for children with information on fat-free mass, allowing all of the candidate predictors to be tested along with their respective non-linear terms while adhering to the 10 people per candidate predictor rule of thumb.30 The wide age range of 4-15 years, including a range of ethnic origins, allowed derivation of a robust model applicable to a wider target population, with consistent performance of the model across the range of age, sex, and ethnic groups. Data collection for all four derivation studies was completed during 2009-13 and should have continuing relevance, with no indication that the associations between fat-free mass and its predictors have changed. We were able to identify an additional independent dataset for external validation, although with a narrower range of age and ethnicity. The model is based on simple and already widely measured predictors. The performance of the model is strong and allows discrimination between fat mass and fat-free mass both in the whole study population and in specific ethnic groups, offering potential advantages over the ethnic specific BMI adjustments that we reported previously,15 particularly if earlier reports suggested that fat mass is more strongly associated with long term health outcomes than is BMI.47 Although the inclusion of non-linear polynomial terms makes the derived algorithm appear complicated for practical use, these terms have been integrated into a simple MS Excel calculator (supplementary file). The derivation of the model was based on the reference standard deuterium dilution method, which provides accurate, safe, and minimally invasive measurements of total body water (and fat-free mass) with an error of less than 1%.4849 Although potential differences might occur in the assessment of total body water and hydration between ethnic groups, previous studies have suggested that the hydration of lean body mass is highly consistent between people50 and that ethnic variations in the hydration of lean body mass are small.51 Moreover, the predictive ability of the final model is strong across the whole study population and does not differ appreciably between ethnic groups. The final prediction models should therefore be widely applicable within the UK population and might also be applicable in a range of other populations, although separate validation studies will be needed before such application.
Implications for clinicians and policymakers
The availability of a prediction model that can accurately assess fat mass in UK children has important potential implications for practice and policy. The model could be used to assess fat mass in individual children as a guide to clinical management, particularly when used as a height standardised indicator. The Excel calculator (supplementary file) would allow simple calculation of fat mass from the relevant predictor variables. An early application could be in the interpretation of routine surveillance of adiposity in children, particularly in the National Child Measurement Programme, in which all the parameters needed for the prediction model are routinely measured. This would allow direct assessment of geographical, ethnic, socioeconomic, and temporal variations in fat mass rather than reliance on weight based measures, which do not distinguish between fat mass and fat-free mass.
Future research should seek to obtain clear evidence on the benefits of this approach compared with conventional weight-for-height measures. It will also require the documentation of normal ranges for the relevant fat mass parameters in different age and sex groups and explore whether body fatness in childhood is more strongly associated than BMI with adult health outcomes, particularly the incidence of type 2 diabetes and cardiovascular disease. Finally, for international applications of the models, further validation in a range of different populations is needed.
What is already known on this topic
Body mass index (BMI), the most widely used marker of body fatness, has serious limitations, particularly in children
As a weight based measure, BMI does not discriminate between lean and fat mass, which can vary greatly in those with a given BMI and might relate differently to risk of cardiometabolic disease
More accurate simple methods, based on routinely available measurements, are needed to improve the assessment of body fatness in childhood
What this study adds
A newly developed and validated prediction model to estimate fat mass levels in UK children aged 4-15 years allows for accurate discrimination of lean and fat mass
The equation is based on readily available markers of height, weight, age, sex, and ethnic group (when available), without the need for more costly forms of assessment
We thank the children who took part in the deuterium dilution studies; the staff involved in recruitment and data collection; the families who took part in the Avon Longitudinal Study of Parents and Children (ALSPAC) study; the midwives for their help in recruiting families; the ALSPAC team, which includes interviewers, computer and laboratory technicians, clerical workers, research scientists, volunteers, managers, receptionists, and nurses; and J J Reilly for helpful advice.
Contributors: MTH, JCKW, RDR, CGO, DGC, ARR, PHW, and CMN designed the study. PHW, CMN, CGO, SL, JEW, DH, MSF, JCKW, ARR, and DGC collected the data. MTH, RDR, ARR, DGC, and CMN analysed the data. MTH, RDR, PHW, ARR, CGO, DGC, and CMN interpreted the data. MTH, PHW, RDR, ARR, and CMN drafted the manuscript. MTH, MSF, DH, SL, JEW, JCKW, RDR, CGO, DGC, ARR, PHW, and CMN critically evaluated and revised the manuscript. The corresponding author attests that all listed authors meet authorship criteria and that no others meeting the criteria have been omitted. This publication is the work of the authors who will serve as guarantors for the contents of this paper.
Funding: This research was supported by grants from the British Heart Foundation (PG/15/19/31336 and FS/17/76/33286). Diabetes prevention research at St George’s, University of London, is supported by the National Institute of Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care South London (NIHR CLAHRC-2013-10022). CMN is supported by the Wellcome Trust Institutional Strategic Support Fund (204809/Z/16/Z) awarded to St George’s, University of London. Data collection in the Assessment of Body Composition in Children study, East London Bioelectrical Impedance, Reference Child, and Size and Lung function in Children studies was funded by the British Heart Foundation (PG/11/42/28895), the BUPA Foundation (TBF-S09-019), Child Growth Foundation (GR 10/03), Wellcome Trust (WT094129MA), and Medical Research Council. The UK Medical Research Council and Wellcome Trust (grant ref 102215/2/13/2) and the University of Bristol provide core support for the Avon Longitudinal Study of Parents and Children study. The views expressed in this paper are those of the authors and not necessarily those of the funding agencies, the National Health Service, the NIHR, or the Department of Health.
Competing interests: All authors have completed the ICMJE uniform disclosure form at www.icmje.org/coi_disclosure.pdf and declare: this research was supported by grants from the British Heart Foundation (PG/15/19/31336 and FS/17/76/33286). Diabetes prevention research at St George’s, University of London, is supported by the National Institute of Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care South London (NIHR CLAHRC-2013-10022); no financial relationships with any organisations that might have an interest in the submitted work in the previous three years; no other relationships or activities that could appear to have influenced the submitted work.
Ethical approval: Ethical approval for the four studies from which data were used to derive the model was obtained from the relevant ethics committees and for the Avon Longitudinal Study of Parents and Children (ALSPAC) study was obtained from the ALSPAC Ethics and Law Committee and the local research ethics committees.
Data sharing: For access to data from the studies used to derive and validate the model contact the study principal investigators (PHW, CMN, and JCKW).
Transparency: The lead author (MTH) affirms that the manuscript is an honest, accurate, and transparent account of the study being reported; that no important aspects of the study have been omitted; and that any discrepancies from the study as planned have been explained.
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