Short communicationFecal recovery following oral administration of Lactobacillus Strain GG (ATCC 53103) in gelatine capsules to healthy volunteers
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Mining Lactobacillus and Bifidobacterium for organisms with long-term gut colonization potential
2020, Clinical NutritionCitation Excerpt :Various methods have been developed to evaluate bacterial colonization in complex gut microbiota (Fig. 1B). In the preliminary stage of research, selective medium with colony identification methods (colony morphology, biochemical methods, 16S rDNA [ribosomal DNA] PCR, internally transcribed spacer [ITS]–PCR, pulsed field gel electrophoresis [PFGE], monoclonal antibodies, and RAPD-PCR) [37,50,57,58,60,68] are generally used. These methods are arduous, time consuming, and of poor accuracy.
Gut feelings: A randomised, triple-blind, placebo-controlled trial of probiotics for depressive symptoms
2019, Journal of Affective DisordersCitation Excerpt :The dosage size of the probiotic may not have been sufficient to be detected in the stool but still have resulted in the psychological effects seen in the study. For example, it was shown that probiotic supplementation of Lactobacillus rhamnosus GG at 108 CFU was detectable in only 1 of 10 faecal samples, however this same strain at a higher dose of 1012 CFU was detected in all 10 faecal samples (Saxelin et al., 1995). The dose used in this study (1010 cfu/day) is above the minimum dose requirement for probiotics without strain specific claims (Hill et al., 2014), and the probiotic supplementation was double that of the dose used with healthy controls in Steenbergen et al. (2015).
Targeted delivery of probiotics to enhance gastrointestinal stability and intestinal colonisation
2017, International Journal of PharmaceuticsCitation Excerpt :For probiotics delivered into the gastrointestinal tract to act, these need to colonise the intestines. Evaluation of probiotic colonisation in vivo is very challenging; a few studies have used the presence of probiotics in faeces as an indication of colonisation; this is, however, very speculative as faecal enumeration generally represents unadhered strains (Saxelin et al., 1993, 1995). An in vitro evaluation was demonstrated here whereby adhesion to Caco-2 intestinal cells was used to evaluate probiotic adhesion.
Efficacy of pasteurised yoghurt in improving chronic constipation: Arandomised, double-blind, placebo-controlled trial
2015, International Dairy JournalCitation Excerpt :Adhesive probiotic has the ability to prevent the attachment of pathogens, for example pathogenic coliform bacteria and clostridia. This role stimulates pathogen removal from an infected intestinal tract (Benno & Mitsuoka, 1992; Lidbeck et al., 1987; Saxelin, Pessi, & Salminen, 1995). This would lead to the higher level of bifidobacteria and lactobacilli in the intestinal canal described here.
Metaanalyse zu präventiven und therapeutischen Effekten probiotischer Supplementierung bei Kindern mit atopischer Dermatitis
2023, JDDG - Journal of the German Society of Dermatology