Summary
By two-dimensional electrophoresis of human serum a genetically determined polymorphism of apolipoprotein E (apoE) can be demonstrated. Three alleles occur with appreciable frequency in Caucasian populations. In the present study the segregation of apoE and complement component C3 (C3) types in material from Norwegian families has been studied. Linkage has convincingly been demonstrated between the two loci with a lod score of 3.00 in males at a recombination fraction of 13%. As it is known that the C3 locus is situated on chromosome 19 in man, apoE can be located to this specific chromosome. Positive linkage data do not, to our knowledge, at present exist with regard to other apolipoproteins.
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Anderson NG, Anderson NL (1978a) Analytical techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: Multiple isoelectric focusing. Anal Biochem 85:331–340
Anderson NL, Anderson NG (1978b) Analytical techniques for cell fractions. XXII. Two-dimensional analysis of serum and tissue proteins: Multiple gradient-slab gel electrophoresis. Anal Biochem 85:341–354
Berg K, Heiberg A (1978) Linkage between familial hypercholesterolemia with xanthomatosis and the C3 polymorphism confirmed. Cytogenet Cell Genet 22:621–623
Børresen A-L, Berg K (1981) The apoE polymorphism studied by two-dimensional, high resolution gel electrophoresis of serum. Clin Genet 20:438–448
Edwards JH, McKusick VA (1982) Cytogenet Cell Genet 32:191–193
Morton NE (1955) Sequential tests for the detection of linkage. Am J Hum Genet 7:277–318
Ott J, Schrott HG, Goldstein JL, Hazzard WR, Allen FH Jr, Falk CT, Motulsky AG (1974) Linkage studies in a large kindred with familial hypercholesterolemia. Am J Hum Genet 26:598–603
Shore B, Shore V (1973) Heterogeneity of human plasma very low density lipoproteins. Separation of species differing in protein components. Biochemistry 12:502–507
Teisberg P (1970) High voltage agarose gel electrophoresis in the study of C3 polymorphism. Vox Sang 19:47–56
Tracy RP, Currie RM, Young DS (1982) Two-dimensional gel electrophoresis of serum specimens from a normal population. Clin Chem 28:890–899
Utermann G, Hees M, Steinmetz A (1977) Polymorphism of apolipoprotein E and occurrence of dysbetalipoproteinemia in man. Nature 269:604–607
Weisgraber KH, Rall SC Jr, Mahley RW (1981) Human E apoprotein heterogeneity: Cysteine-arginine interchanges in the amino acid sequence of the apo-E isoforms. J Biol Chem 256:9077–9081
Whitehead AS, Solomon E, Chambers SP, Povey S, Bodmer WF (1982) Assignment of the gene for the third component of human complement (C3) to chromosome 19 using human mouse somatic cell hybrids. Cytogenet Cell Genet 32:326
Weitkamp LR, Johnston E, Guttormsen SA (1974) Probable genetic linkage between the loci for the Lewis blood group and complement C3. Cytogenet Cell Genet 13:183–184
Zannis VJ, Just PW, Breslow JL (1981) Human apolipoprotein E isoprotein subclasses are genetically determined. Am J Hum Genet 33:11–24
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Olaisen, B., Teisberg, P. & Gedde-Dahl, T. The locus for apolipoprotein E (apoE) is linked to the complement component C3 (C3) locus on chromosome 19 in man. Hum Genet 62, 233–236 (1982). https://doi.org/10.1007/BF00333526
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DOI: https://doi.org/10.1007/BF00333526