Antenatal lifestyle advice for women who are overweight or obese: LIMIT randomised trialBMJ 2014; 348 doi: http://dx.doi.org/10.1136/bmj.g1285 (Published 10 February 2014) Cite this as: BMJ 2014;348:g1285
- Jodie M Dodd, professor of obstetrics and gynaecology; maternal fetal medicine specialist12,
- Deborah Turnbull, professor of psychology3,
- Andrew J McPhee, director of neonatal medicine4,
- Andrea R Deussen, senior clinical trials coordinator1,
- Rosalie M Grivell, senior lecturer in obstetrics and gynaecology; maternal fetal medicine specialist12,
- Lisa N Yelland, senior statistician1,
- Caroline A Crowther, professor of obstetrics and gynaecology1,
- Gary Wittert, professor medicine5,
- Julie A Owens, head of school paediatrics and reproductive health; associate dean, research1,
- Jeffrey S Robinson, professor of obstetrics and gynaecology1
- for the LIMIT Randomised Trial Group
- 1Robinson Institute and School of Paediatrics and Reproductive Health, University of Adelaide, 72 King William Road, North Adelaide, South Australia, Australia 5006
- 2Department of Perinatal Medicine, Women’s and Babies Division, Women’s and Children’s Hospital, 72 King William Road, North Adelaide, South Australia, Australia 5006
- 3School of Psychology, University of Adelaide, Adelaide, South Australia, Australia 5005
- 4Department of Neonatal Medicine, Women’s and Babies Division, Women’s and Children’s Hospital, 72 King William Road, North Adelaide, South Australia, Australia 5006
- 5School of Medicine, University of Adelaide, Adelaide, South Australia, AUSTRALIA 5005
- Correspondence to: J M Dodd
- Accepted 24 January 2014
Objective To determine the effect of antenatal dietary and lifestyle interventions on health outcomes in overweight and obese pregnant women.
Design Multicentre randomised trial. We utilised a central telephone randomisation server, with computer generated schedule, balanced variable blocks, and stratification for parity, body mass index (BMI) category, and hospital.
Setting Three public maternity hospitals across South Australia.
Participants 2212 women with a singleton pregnancy, between 10+0 and 20+0 weeks’ gestation, and BMI ≥25.
Interventions 1108 women were randomised to a comprehensive dietary and lifestyle intervention delivered by research staff; 1104 were randomised to standard care and received pregnancy care according to local guidelines, which did not include such information.
Main outcome measures Incidence of infants born large for gestational age (birth weight ≥90th centile for gestation and sex). Prespecified secondary outcomes included birth weight >4000 g, hypertension, pre-eclampsia, and gestational diabetes. Analyses used intention to treat principles.
Results 2152 women and 2142 liveborn infants were included in the analyses. The risk of the infant being large for gestational age was not significantly different in the two groups (lifestyle advice 203/1075 (19%) v standard care 224/1067 (21%); adjusted relative risk 0.90, 95% confidence interval 0.77 to 1.07; P=0.24). Infants born to women after lifestyle advice were significantly less likely to have birth weight above 4000 g (lifestyle advice 164/1075 (15%) v standard care 201/1067 (19%); 0.82, 0.68 to 0.99; number needed to treat (NNT) 28, 15 to 263; P=0.04). There were no differences in maternal pregnancy and birth outcomes between the two treatment groups.
Conclusions For women who were overweight or obese, the antenatal lifestyle advice used in this study did not reduce the risk delivering a baby weighing above the 90th centile for gestational age and sex or improve maternal pregnancy and birth outcomes.
Trial registration Australian and New Zealand Clinical Trials Registry (ACTRN12607000161426).
The World Health Organization has identified obesity as a health problem of global importance,1 contributing to the burden of disease through an increased risk of cardiovascular disease and diabetes and their complications.2 Overweight and obesity are commonly encountered during pregnancy, with about half of women entering pregnancy with a body mass index (BMI) ≥25.3 4
The risks associated with overweight and obesity during pregnancy are well documented and increase with increasing BMI.5 6 Maternal complications include an increased risk of hypertension and pre-eclampsia,5 6 7 gestational diabetes,5 6 7 need for both induction of labour and caesarean section,5 6 7 and perinatal death.8 9 Infants born to overweight or obese women are more likely to be macrosomic,6 7 8 9 be admitted to intensive care,5 6 9 and require treatment for jaundice or hypoglycaemia.5 6 Furthermore, population studies consistently identify both maternal overweight and obesity and high infant birth weight (variably defined as birth weight above the 90th centile for gestational age or birth weight above 4000 g or 4500 g) as important risk factors for the development of early infant10 and childhood obesity.11
Substantial literature on maternal weight gain in pregnancy has been summarised by the Institute of Medicine.12 Several population based cohort studies have evaluated the effect of varying degrees of gestational weight gain on pregnancy outcomes for women who are overweight or obese, suggesting that, compared with higher weight gain, weight gain within the recommendations of the Institute of Medicine is associated with a reduced risk of maternal complications and of infants being born large for gestational age.13 14 15 16 Most small randomised trials to date evaluating antenatal dietary and lifestyle interventions in women who are overweight or obese have primarily focused on limiting gestational weight gain, often as a surrogate measure for clinical outcomes, based on the assumption that lower weight gain will in turn improve outcomes in pregnancy and childbirth. While meta-analyses indicate that interventions seem effective in limiting gestational weight gain, the effect on maternal and perinatal outcomes, which have been under-reported, remains to be determined.17 18 19
We conducted a randomised trial to evaluate whether provision of antenatal lifestyle advice to overweight or obese pregnant women was effective in improving maternal and infant health outcomes.
Women were recruited between June 2008 and December 2011 from the three major metropolitan maternity hospitals within Adelaide, South Australia. Women were eligible if they had a BMI ≥25 and singleton pregnancy at 10+0 to 20+0 weeks’ gestation. Women with type 1 or 2 diabetes diagnosed before pregnancy were ineligible. Each participant provided written informed consent, and the ethics committee at each collaborating hospital approved the protocol.20
Randomisation, allocation concealment, and blinding
All pregnant women presenting for antenatal care at the participating centres had their height and weight measured and BMI calculated at their first antenatal appointment. A research assistant counselled eligible women and then randomised them to receive lifestyle advice or standard care by telephoning the central randomisation service. The computer generated randomisation schedule used balanced variable blocks in the ratio 1:1 and was prepared by an investigator not involved with recruitment or clinical care. Stratification occurred for parity (0 v ≥1), BMI at antenatal booking (25-29.9 v ≥30), and collaborating centre. Outcome assessors were blinded to the treatment group allocated.
Women randomised to lifestyle advice participated in a comprehensive dietary and lifestyle intervention over the remainder of their pregnancy that included a combination of dietary, exercise, and behavioural strategies delivered by a research dietician and trained research assistants.20 Women were provided with dietary advice consistent with current Australian standards21 to maintain a balance of carbohydrates, fat, and protein and to reduce intake of foods high in refined carbohydrates and saturated fats, while increasing intake of fibre and promoting consumption of two servings of fruit, five servings of vegetables, and three servings of dairy each day.21 Physical activity advice primarily encouraged women to increase their amount of walking and incidental activity.22 Tailoring of the intervention was informed by stage theories of health decision making.23
Within two weeks of randomisation, women attended a planning session with a research dietician, during which a detailed dietary and exercise history was obtained. Women were provided with individualised information, including meal plans, healthy recipes that were quick to prepare, simple food substitutions (including reducing sugar sweetened soft drinks and fruit juices, reducing added sugar and foods high in refined carbohydrates, and low fat alternatives), options for healthy snacking and eating out, and guidelines for healthy food preparation. Women were encouraged to set achievable goals for dietary and exercise change, supported to make these lifestyle changes, and asked to self monitor their progress through the use of a work book provided. Women were encouraged to identify potential barriers to implementation of their dietary and physical activity goals. Using these perceived barriers, women were assisted to problem solve and to develop individualised strategies to facilitate their successful implementation. This information was reinforced during subsequent inputs provided by the research dietician (at 28 weeks’ gestation) and trained research assistants (via telephone call at 22, 24, and 32 weeks’ gestation and a face to face visit at 36 weeks’ gestation).
Women randomised to standard care continued to receive their pregnancy care according to state-wide perinatal practice and local hospital guidelines, which during the course of the trial did not include routine provision of advice related to diet, exercise, or gestational weight gain.24
Follow-up of women in both groups
All women had their ongoing clinical care provided by clinicians at their planned hospital of birth. Consistent with state-wide perinatal practices, all women were encouraged to undergo screening for gestational diabetes.25 After birth, a research assistant not involved in providing the intervention and blinded to treatment allocation obtained information relating to antenatal, birth, and infant outcomes from the case notes.
The primary outcome was the incidence of infants born large for gestational age (birth weight ≥90th centile for gestational age and infant sex).26 While large for gestational age, high infant birth weight, and infant macrosomia (variably defined as birth weight above 4000 g or 4500 g) are often used clinically, incorporation of a birthweight centile for gestational age was considered important, reflecting the possibility that the intervention might modify fetal growth across pregnancy and therefore plausibly mediate high infant birth weight across the gestational age spectrum.
Prespecified secondary infant outcomes included preterm birth (before 37 weeks); mortality (stillbirth (intrauterine fetal death after 20 weeks’ gestation and before birth), or infant death (death of a liveborn infant before hospital discharge, excluding lethal congenital anomalies)); weight >4000 g; hypoglycaemia requiring intravenous treatment; admission to neonatal intensive care unit or special care baby unit; hyperbilirubinaemia requiring phototherapy; nerve palsy (peripheral nerve injury, including spinal cord injury, present at discharge from hospital); fracture (basal skull or other fracture identified at birth); birth trauma (subdural or intracerebral haemorrhage, spinal cord injury, or peripheral nerve injury present at hospital discharge); or shoulder dystocia (one or more of moderate traction, suprapubic pressure, lithotomy position, McRoberts manoeuvre, rotation into the oblique plane, delivery of the posterior shoulder, cleidotomy, symphysiotomy, or Zavanelli manoeuvre to deliver the fetal shoulders).
Prespecified secondary maternal outcomes included hypertension (systolic blood pressure ≥140 mm Hg and/or diastolic ≥90 mm Hg on two occasions four or more hours apart) and pre-eclampsia27; gestational diabetes (positive 75g oral glucose tolerance test result with fasting blood glucose ≥5.5 mmol/L or 2 hour ≥7.8 mmol/L)25; need for and length of antepartum hospital stay; antepartum haemorrhage requiring stay in hospital; preterm ruptured membranes before labour; term ruptured membranes before labour; chorioamnionitis requiring antibiotics during labour; need for induction of labour; antibiotic use during labour; caesarean section; postpartum haemorrhage (>600 mL); perineal trauma; wound infection; endometritis; antibiotics postpartum; postpartum length of hospital stay; thromboembolic disease; and maternal death.
In a post hoc analysis, we categorised gestational weight gain (defined as the difference in weight measured between 36 weeks’ gestation (or closest to birth) and the antenatal booking visit) as the proportion of women whose weight gain was below, within, or exceeded the Institute of Medicine recommendations based on early BMI category.12
Analyses were performed on an intention to treat basis, according to the treatment group allocated at randomisation. Multiple imputation was performed separately by treatment group with chained equations to create 100 complete datasets. Sensitivity analyses with the available data and different imputation models produced similar results. Binary outcomes were analysed with log binomial regression, with treatment effects expressed as relative risks, or Fisher’s exact tests. Continuous outcomes were analysed with linear regression, with treatment effects expressed as differences in means. Count outcomes were analysed with Poisson regression, or negative binomial regression where overdispersion was present, with treatment effects expressed as ratios of means.
Both unadjusted and adjusted analyses were performed, with adjustment for the stratification variables. Outcomes derived from birth weight were additionally adjusted for maternal age, socioeconomic status, and maternal smoking. Significance was set at P<0.05 (two sided) with no adjustment for multiple comparisons. All analyses followed a prespecified analysis plan with SAS v9.3 (Cary, NC, US). Post hoc outcomes are clearly identified and no imputation was performed.
We estimated that a sample size of 2180 women would have 80% statistical power (two tailed α level 0.05) to detect a 30% relative reduction28 in the occurrence of infants born large for gestational age from 14.4% to 10.1%, allowing for 15% attrition.3
The figure outlines the flow of participants in the trial⇓. A total of 2152 women and 2142 liveborn infants were included in the intention to treat analyses. There were two maternal deaths (motor vehicle collision in lifestyle advice group; and ruptured maternal splenic artery aneurysm in standard care group). There were 10 stillborn infants (two from placental abruption, one from severe intrauterine growth restriction, one from acute chorioamnionitis, and one from motor vehicle collision in lifestyle advice group; and two from placental insufficiency, one from obstetric cholestasis, and two unexplained in standard care group) and two neonatal deaths (excluding lethal congenital anomalies) from complications of extreme prematurity, one in each group.
Table 1⇓ outlines the baseline demographics and clinical characteristics of participants. The median BMI of the cohort was 31.1 (interquartile range 27.9-35.8), with 42.1% of women being overweight and 57.9% obese. Of women randomised to lifestyle advice, 86.7% attended their first dietary appointment and 77.2% the second. Adequate data were available for 2127 (99.3%) infants for the primary outcome. Missing values for the 15 remaining infants were imputed as described above.
There was no significant difference in the risk of infants born large for gestational age in the lifestyle advice group compared with standard care group (203/1075 (19%) v 224/1067 (21%); adjusted relative risk 0.90, 95% confidence interval 0.77 to 1.07; P=0.24) (table 2⇓). Infants born to women allocated to lifestyle advice, however, were less likely to weigh above 4000 g (164/1075 (15%) v 201/1067 (19%); 0.82, 0.68 to 0.99; number needed to treat (NNT) 28, 15 to 263; P=0.04) when compared with infants born to women allocated to standard care.
There was no significant difference in preterm birth before 37 weeks or infant admission to neonatal intensive care or special care unit (table 2⇑). For women, there were no significant differences in risk of antenatal (table 3)⇓, labour, or birth (table 4)⇓ complications after the lifestyle intervention compared with standard care.
In a post hoc analysis, there was no difference in total gestational weight gain (9.39 (SD 5.74) kg in the lifestyle advice group v 9.44 (SD 5.77) kg in standard care; adjusted mean difference −0.04, −0.55 to 0.48; P=0.89) between the two groups (table 5⇓). Furthermore, there were no significant differences between the two treatment groups in the proportion of women whose weight gain was below, within, or exceeded the Institute of Medicine recommendations based on BMI in early pregnancy (table 5⇓).12
Our findings indicate that provision of lifestyle advice to women who are overweight or obese during pregnancy does not reduce the risk of infants born large for gestational age or improve maternal outcomes of pregnancy and birth but is associated with a significant reduction in the risk of birth weight above 4000 g.
Strengths and limitations
Our randomised trial has several strengths, including being the largest to date to evaluate the effect of an antenatal lifestyle intervention for overweight or obese women. Our trial used robust methods, including prospectively measured height, weight, and BMI in all participants, central randomisation, and blinding of outcome assessors. Furthermore, the trial had a prespecified sample size that was achieved, had prespecified primary and secondary outcomes of clinical relevance, followed a prespecified analysis plan, and achieved a high rate of follow-up of participants. We had few exclusion criteria, and the intensity of the intervention was realistically achievable within current publicly funded models of provision of antenatal care.
In contrast with the findings reported in systematic reviews evaluating dietary interventions in overweight or obese pregnant women,17 18 19 which included smaller randomised trials, often of low methodological quality, we did not identify differences in mean gestational weight gain. While there has been considerable variation in the intensity of interventions provided across trials,17 18 19 ranging from single sessions with a dietician up to additional dietetic counselling sessions at each antenatal visit, most trials provided between three and six intervention sessions over the course of pregnancy. The intensity of our intervention was consistent with this approach, involving three face to face sessions and three telephone contacts and, importantly, was considered feasible to implement within current publicly funded models of maternity care. While increasing the intensity of the intervention might have resulted in differences in gestational weight gain, we consider this an impractical approach, both from a healthcare perspective where resources are limited, and from an individual woman’s perspective where additional attendance for visits could further affect compliance. Furthermore, it will be important to assess the economic costs associated with providing the described moderate intensity intervention in our planned detailed evaluation.
A potential limitation of our trial is the generalisability and external validity of our findings, our population being predominantly white and of high social disadvantage, with 60% of eligible women declining to participate, reflecting both a lack of interest and time because of other commitments. Furthermore, not all women attended scheduled appointments with the dietitian, with only 87% attending their first and 77% their second dietary sessions. Compliance with intervention in trials involving overweight and obese pregnant women has been poorly reported in the literature to date.17 18 19 Of note, the Danish randomised trial by Vinter and colleagues29 reported higher rates of attendance than were achieved in our trial, probably reflecting differences in characteristics of the study population, the women being of higher socioeconomic and educational status than the participants in our trial.
Comparison with other studies
Our findings highlight the difficulties in limiting gestational weight gain at a population level, with 42% of women gaining in excess of the Institute of Medicine recommendations.12 Research efforts to date have focused on limiting gestational weight gain, to the exclusion of adequate evaluation of relevant maternal and perinatal outcomes, based on a plausible assumption, although relatively untested by randomised trials, that lower weight gain results in improved outcomes in pregnancy and childbirth. Gestational weight gain as a measure incorporates the effects of maternal fat deposition, pregnancy related plasma volume expansion, peripheral oedema, placental mass, fetal mass, and amniotic fluid volume. It is difficult, however, to separate the relative contribution of each of these components, with maternal deposition of adipose tissue likely to have the greatest causal impact in terms of both short term pregnancy related outcomes and the longer term health of the child.30
While the effect of any changes in maternal dietary intake, including specific nutritional components, and physical activity on in utero growth remains to be determined, observational studies suggest that consumption of a diet high in polyunsaturated fatty acids is associated with reduced early childhood adiposity as measured by skinfold thickness31 and predictive of fat mass determined by DEXA at ages 4 and 6 years.32 Together, these findings highlight the potential impact of relatively modest changes in the quality of maternal diet on in utero growth and future childhood adiposity.
We did not find a difference in the risk of infants born large for gestational age, although our intervention was associated with a significant reduction in the risk of an infant being born weighing above 4000 g. This reduction in the proportion of infants with birth weight above 4000 g could reflect a chance event, which occurred despite a non-significant reduction in the risk of preterm birth and a non-significant increase in gestational diabetes for women receiving lifestyle advice. Alternately, however, these findings are consistent with those of Sebire and colleagues,33 who, using a large UK population based cohort, showed that while infants born to overweight or obese women were more likely to have weight at the upper end of the spectrum, there was little change in the remainder of the distribution compared with infants born to women of normal BMI.33 While this is similar to the birthweight findings in our standard care group, it is plausible that our intervention successfully reduced the proportion of infants with high birth weight. As infant birth weight above 4000 g was a secondary outcome in our trial, it will be important to evaluate this finding in ongoing randomised trials evaluating the effect of dietary interventions in obese women.34
The immediate consequences of infant macrosomia and its associated effects on delivery are well described.35 There is, however, increasing evidence of a persisting longer term health legacy, with birth weight above 4000 g independently associated with an increased risk of overweight and obesity in childhood and adulthood.36 37 Specifically, birth weight greater than 4000 g has been associated with a twofold increased risk of obesity at age 4 years38 39 and beyond,36 37 highlighting the public health importance of high birth weight and of evaluating interventions that target the in utero environment in the short and long term prevention of obesity.40 It will therefore be important to continue to follow-up the infants born to women in this trial to evaluate if there is any effect of the observed 18% relative risk reduction in incidence of birth weight above 4000 g on subsequent early childhood obesity.
Conclusions and policy implications
To date, there has been a lack of robust clinical outcome data from randomised trials on the effects of providing an antenatal dietary and lifestyle intervention for pregnant women who are overweight or obese, the research focus being on “gestational weight gain, at the expense of adequate power for clinical outcomes.”41 Our trial dealt with this highlighted gap in the literature. Our results indicate that while provision of an antenatal dietary and lifestyle intervention did not reduce the incidence of infants born large for gestational age, it was effective in reducing the risk of high infant birth weight, above 4000 g. The intervention provided was not associated with improvements in the various maternal pregnancy and birth outcomes, which are documented to occur more commonly among women who are overweight or obese. While a more intensive intervention might be effective in modifying these risks, there are important considerations both in relation to the cost of providing such an intervention and maternal compliance, which might impact feasibility. Alternate approaches might entail the use of drugs, including metformin, which is currently being evaluated.42 43
What is already known on this topic
Overweight and obesity is common during pregnancy
Increasing maternal BMI and high gestational weight gain are both associated with an increased risk of complications during pregnancy and childbirth
Systematic reviews have indicated that while antenatal dietary and lifestyle interventions seem effective in limiting gestational weight gain, the effect on clinically relevant outcomes remains to be determined
What this study adds
The findings of this randomised trial indicate that providing a dietary and lifestyle intervention to pregnant women who are overweight or obese was not associated with a reduction in the incidence of infants born large for gestational age or in improving maternal pregnancy and birth outcomes
Cite this as: BMJ 2014;348:g1285
We are indebted to all the women who participated in this randomised trial.
The following people and institutions (in Adelaide, South Australia, unless indicated otherwise) participated in the LIMIT Trial:
Steering group—JMD, DT, AMcP, RMG, CAC, M Gillman (Obesity Prevention Program, and Harvard University, Boston, Massachusetts, US), GW, JAO, JSR.
Coordinating team—JMD, ARD, RMG, LNY, L Moran, C Cramp, A Newman, L Kannieappian, S Hendrijanto, M Kelsey, J Beaumont, C Danz, J Koch, A Webber, C Holst, K Robinson, S Zhang, V Ball, K Ball, H Deussen, N Salehi, R Bartley, R Stafford-Green, S Ophel, M Cooney, M Szmeja, A Short, A Melrose, S Han, I Mohamad, L Chapple.
Adverse events committee—RMG, J Svigos, V Bhatia, N Manton.
Collaborating Hospitals (total number of women recruited from each site in parentheses), *indicates named associate investigator for the NHMRC grant.
Flinders Medical Centre (South Australia) (n=669): J McGavigan*, R Bryce, S Coppi, C Fanning, G Hannah, M Ignacio, H Pollard, F Schmidt, Y Shinners
Lyell McEwin Hospital (South Australia) (n=505): G Dekker*, S Kennedy-Andrews, R Beaven, J Niven, S Burgen, J Dalton, N Dewhurst, L Forst, V Mugg, C Will, H Stone
Women’s and Children’s Hospital (South Australia) (n=1038): JMD, JSR, ARD, CAC*, C Wilkinson*, H Purcell, J Wood, D Press, K Ralph, S Donleavy, S Seager, F Gately, A Jolly, L Lahnstein, S Harding, K Daw, M Hedges, R Fraser-Trumble
Contributors: All authors were involved equally in the development and design of the trial, the conduct of the trial, drafting of the manuscript, and revision for intellectual content and approved the final submitted version. LNY carried out the statistical analyses. JMD is guarantor.
Funding: This project was funded by a four year project grant from the National Health and Medical Research Council (NHMRC), Australia (ID 519240). JMD is supported through a NHMRC Practitioner Fellowship (ID 627005). The funder had no role in the study design, data collection, analysis, interpretation, or writing of the report.
Competing interests: All authors have completed the ICMJE uniform disclosure form at www.icmje.org/coi_disclosure.pdf and declare: no support from any organisation for the submitted work; no financial relationships with any organisations that might have an interest in the submitted work in the previous three years; no other relationships or activities that could appear to have influenced the submitted work.
Ethical approval: This study was approved by each of the collaborating sites (Research Secretariat, Women’s and Children’s Hospital (application REC 1839/6/2012); Ethics of Human Research Committee, The Queen Elizabeth Hospital and Lyell McEwin Hospital (application 2008033); and Flinders Clinical Research Ethics Committee, Flinders Medical Centre (Application 128/08)). Each participant provided written informed consent,
Data sharing: No additional data available.
Transparency: The lead author and manuscript guarantor (JMD) affirms that the manuscript is an honest, accurate, and transparent account of the study being reported; that no important aspects of the study have been omitted; and that any discrepancies from the study as planned and registered have been explained.
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