Biochemical pathway for the metabolism of methanol. Methanol is metabolised by alcohol dehydrogenase and formaldehyde dehydrogenase to form formate. Both oxidative steps depend on the reduction of the cofactor NAD+ (oxidised nicotinamide adenine dinucleotide) to NADH (the reduced form of nicotinamide adenine dinucleotide). This cofactor is oxidised by the reduction of pyruvate to lactate. Formate is combined with tetrahyrofolate by ATP-dependent reaction catalysed tetrahyrofolate synthetase to form 10-formyl tetrahyrofolate, which is ultimately converted to carbon dioxide and water by 10-formyl tetrahydrofolate dehydrogenase