Research Article

Malaria diagnosis by enzyme-linked immunosorbent assays.

Br Med J (Clin Res Ed) 1981; 282 doi: https://doi.org/10.1136/bmj.282.6278.1747 (Published 30 May 1981) Cite this as: Br Med J (Clin Res Ed) 1981;282:1747
  1. D E Bidwell,
  2. A Voller

    Abstract

    Enzyme-linked immunosorbent assays (ELISA) for detecting malarial parasites in blood were tested on uninfected monkeys and in monkeys infected with Plasmodium falciparum. A double antibody sandwich ELISA detected one malarial cell per 10(3) uninfected cells, and an inhibition ELISA detected on infected cell per 10(4) uninfected erythrocytes. These methods are not yet as sensitive as conventional blood-film examinations, in which a well-trained microscopist might be expected to detect one malarial cell per 10(6) erythrocytes. Nevertheless, ELISA offers an objective means of detecting malaria and is particularly advantageous since up to 100 samples may be processed simultaneously.