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New In-vitro Test for IgE-mediated Hypersensitivity in Man

Br Med J 1972; 2 doi: https://doi.org/10.1136/bmj.2.5814.623 (Published 10 June 1972) Cite this as: Br Med J 1972;2:623

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  1. Malcolm W. Greaves,
  2. Shoso Yamamoto,
  3. Valerie M. Fairley

    Abstract

    A new simple and sensitive in-vitro method for the diagnosis of type 1 (IgE-mediated) hypersensitivity in man is described. Sliced human skin is passively sensitized by reaginic serum from allergic patients and the presence of antigen-specific IgE on the sensitized slices is detected by assay of antigen-evoked histamine release. Serum from 12 out of 14 patients with clinical respiratory allergy and positive skin tests gave significant antigen-specific histamine release. This method, which is essentially an in-vitro model of the Prausnitz-Küstner reaction, should prove of value in the diagnosis of human reaginic hypersensitivity in man.

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