Introduction

Top Abstract Introduction Methods Results Discussion References

We reported an increased prevalence of complex endometrial hyperplasia in postmenopausal women receiving standard sequential oestrogen-progestogen hormone replacement therapy compared with those receiving no hormone replacement therapy. ^{1 2} Other studies reported an increased risk of endometrial carcinoma in women who used such treatments over long periods compared with those who did not. ^{3 4} Information about the safety of standard sequential oestrogen-progestogen hormone replacement therapy used over long periods, along with a lack of compliance because of withdrawal bleeding, has highlighted the need for alternative types of hormone replacement therapy. ^{5 6}

Continuous combined hormone replacement therapy, in which oestrogen and progestogen are given together daily, is being used more often. Such regimens avoid the cyclical problems associated with sequential therapy in most women and combine protection of the endometrium with amenorrhoea, so that compliance improves.^7-11 Safety data about such regimens are needed if long term treatment is to be recommended, but such data are limited. Data that are available have looked at periods of use only up to three years. ^{12 13}

The endometrium becomes atrophic within six months of women starting continuous combined hormone replacement therapy containing 2 mg 17-oestradiol and 1 mg norethisterone acetate.^14-16 In many cases, the endometrium cannot be assessed because biopsy specimens contain insufficient tissue. ^{2 8 17} Continuous combined hormone replacement therapy stops bleeding in most postmenopausal women, ^{2 14-16} and it may transform hyperplastic endometrium into states of atrophy after three to nine months. ^{2 8}

We investigated the effects of continuous combined hormone replacement therapy containing 2 mg 17-oestradiol and 1 mg norethisterone acetate on endometrial histology and bleeding patterns in postmenopausal women treated for up to five years. As far as we are aware, this is the largest long term study of its kind, reporting data from 534 women.

	Methods

Top Abstract Introduction Methods Results Discussion References

An open, prospective, multicentre British study investigated women treated with a continuous combined hormone replacement therapy regimen containing 2 mg 17-oestradiol and 1 mg norethisterone acetate (Kliofem, Novo Nordisk; Kliogest outside the United Kingdom) for nine months.² The study was conducted at 54 menopause clinics. When the study ended, we recruited women who had completed the treatment period and who elected to continue the treatment for up to five years into a long term follow up study. The five year follow up included the nine months of the original study. Women were recruited from 31 of the clinics (29 based in hospitals; two in general practice) that participated in the original trial.

The follow up study was approved by local ethics committees, and it was conducted in accordance with the Declaration of Helsinki (Hong Kong revision, 1989). All women gave written, informed consent to participate.

We took endometrial aspiration biopsies before continuous combined hormone replacement therapy was started, after nine and 24-36 months of treatment, and after five years of treatment or withdrawal from the study. Biopsy specimens were taken in outpatient clinics, using Pipelle de Cornier samplers (Eurosurgical, Guildford).¹⁸ Samples were fixed in 4% unbuffered formalin and processed routinely. A single independent pathologist (MW), who was blind to any details of the patients, assessed the histological samples and classified them according to standard criteria (box 1). We used frequency counts to summarise the results of endometrial biopsies for each type of response. Other data were summarised with appropriate summary statistics.

	Results

Top Abstract Introduction Methods Results Discussion References

Overall, 534 women provided data for the follow up study. Before the original study, 360 women had taken sequential hormone replacement therapy, 164 no hormone replacement therapy, and 10 unopposed oestrogen. The characteristics were similar for women from the different treatment regimens, so the data were pooled (table 1). The mean duration of treatment with continuous combined hormone replacement therapy was 4.4 (range 1.1-5.9 months).

Data about endometrial biopsies were available for 531 women before treatment (table 2), 526 women after nine months of continuous combined hormone replacement therapy (as part of the original study period), 465 (87%) women after 24-36 months (table 3), and 398 (75%) women after 5 years of treatment (345 women) or on withdrawal (53 women). No cases of endometrial hyperplasia or malignancy were detected in women whose biopsy specimens were assessable. Most specimens in the "other" category were benign endocervical or endometrial polyps (figure).

View larger version (19K): [in this window] [in a new window]   Results of histological examination of endometrium from women treated for up to five years with continuous combined hormone replacement therapy

Complex hyperplasia was detected in 21 women who had been taking sequential hormone replacement therapy and one woman who had been taking unopposed oestrogen before the study (table 2). Sixteen of these women completed five years of treatment with continuous combined hormone replacement therapy and six withdrew from the study. In all 22 cases, the complex hyperplasia reverted to normal endometrial patterns after nine months of treatment,² and hyperplasia had not recurred by completion of or withdrawal from the study (table 4). We did not analyse other possible changes in histology from one category of normal histology to another during the study.

	Discussion

Top Abstract Introduction Methods Results Discussion References

No cases of endometrial hyperplasia or malignancy occurred during the five years of the study. We previously showed that all women who had complex hyperplasia while taking previous sequential or unopposed hormone replacement therapy reverted to normal after nine months of continuous combined hormone replacement therapy.² The current study showed that hyperplasia did not recur and that new cases did not develop in women treated for up to five years. The results give reassurance about the long term endometrial safety of this regimen.

Strengths and weaknesses of the study
Most previous long term studies involved small numbers of patients or a shorter duration of treatment, or both. ^{7-10 12 13 17 19} This study confirms that long term treatment for up to five years with a continuous combined hormone replacement therapy regimen of oestradiol 2 mg and norethisterone acetate 1 mg daily was associated with neither endometrial hyperplasia nor malignancy and that it converted the endometrium to normal in women in whom complex hyperplasia arose during previous sequential or unopposed oestrogen regimens.

Strengths and weaknesses in relation to other studies
Our results support the findings of previous studies with long term continuous combined hormone replacement therapy. ^{9 12 13} Staland also found that the combination of oestradiol 2 mg with norethisterone acetate 1 mg was associated with reversion of pre-existing hyperplasia to normal endometrium.²⁰ A recent population based study has reported that women taking continuous combined hormone replacement therapy did not seem to be at any increased risk of endometrial cancer relative to women who had never received hormone replacement therapy.²¹

Endometrial carcinoma has been reported only rarely in women taking continuous combined hormone replacement therapy regimens. ^{10 19 22} In most cases the women had a history of unopposed oestrogen or of sequential use with less than 10 days of progestogen, risk factors such as a family history of endometrial carcinoma, or an inadequate dose of progestogen.²³

The proportion of unassessable biopsies was lower than in previous studies of continuous combined hormone replacement therapy, in which more than 50% of biopsy specimens obtained from postmenopausal women did not have sufficient tissue for assessment. ^{16 17 24 25} A separate study of 40 postmenopausal women treated with the same drug at one of the participating sites confirmed that the Pipelle method of sampling was effective and that an unassessable specimen was associated with endometrial atrophy.²⁶

Most studies did not identify the proportion of endometrial biopsies with secretory or proliferative changes but classified such biopsies along with others in a normal category. A study by Nand et al in 568 women showed atrophic endometria in 95.7-97.9% of women and secretory changes in only 0-1.5%.²⁷ We can only speculate on the reasons for such a difference in the proportion of biopsies with secretory or atrophic changes compared with those in our study (secretory 26%, atrophic 46%), but the populations, regimens, and types of oestrogen and progestogen as well as the histopathologists were all different.

In the PEPI (postmenopausal estrogen-progestin interventions) trial, which lasted three years, 3.4% of 352 women treated with sequential hormone replacement therapy developed simple hyperplasia and 1.7% complex hyperplasiathis gives a total of 5.1% of women who developed hyperplasia over three years.¹² In the group of women who received continuous combined hormone replacement therapy, one case of simple hyperplasia (0.8%) occurred; the remaining 119 women had normal histological results at the end of three years. These findings are comparable with those of our preliminary study and the data presented here.²

Our findings compare favourably with those of Archer et al, who reported on endometrial histology in 801 perimenopausal and postmenopausal women without symptoms.²⁸ Atrophic endometrial tissue was seen in 46.9% of women, proliferative changes in 16.7%, secretory changes in 6.8%, and hyperplasia in 5.2%; cellular atypia and carcinoma were each seen in one case (0.1%).²⁸ Insufficient tissue was found in 24.5% of samples. In our findings, all endometrial tissue was histologically normal during long term continuous combined hormone replacement therapy, suggesting that this type of hormone replacement therapy may protect the endometrium from spontaneous abnormal changes from which untreated women are at risk.

Meaning of the study
Interest in the use of hormone replacement therapy has increased recently. Many women are considering using such therapy over long periods to try to affect their long term healthparticularly in relation to preventing osteoporosis. Women and their doctors need to be reassured that the long term use of hormone replacement therapy will not increase the risk of endometrial cancer, because recent reports showed that this risk may be increased after more than five years of use of sequential oestrogen and progestogen regimens (odds ratios 2.7 (95% confidence interval 1.2 to 6.0) and 2.9 (1.8 to 4.6), respectively), ^{3 4} but not with continuous combined hormone replacement therapy (0.2 (0.1 to 1.8)).⁴ The duration of progestogen treatment in each cycle in sequential regimens is critical: the risk of endometrial cancer is greater when progestogen is taken for less than 10 days. ^{3 29}

In addition to the length of the progestogen phase, the dose and type of progestogen may be important factors. A recent study by Weiderpass and colleagues showed lower rates of endometrial cancer in women treated with continuous combined hormone replacement therapy that included a 19-nortestosterone derivative as the progestogen component than in women who took continuous combined hormone replacement therapy in which a 17-hydroxy-progesterone derivative was the progestogen component.⁴ A study in which women mainly took continuous combined conjugated oestrogens and 17-hydroxy-progesterone derivatives showed no differences in the incidence of endometrial cancer.²⁹ The current study used a continuous combined preparation similar to that taken by most women in the study by Weiderpass et al.⁴ The reversal of endometrial hyperplasia (with no recurrence during five years of follow up) seen in our study may relate to this specific combination, which has a relatively strong progestogen component.

This is the largest and longest study of continuous combined hormone replacement therapy to date. In addition, our study is unique in that it includes women with endometrial hyperplasia. The findings of normal histology, together with the reversion of hyperplasia without cytological abnormality, suggest that continuous daily progestogen in such regimens provides greater protection of the endometrium and reassurance that long term treatment does not harm the endometrium.

Unanswered questions and future research
Despite the reassurance provided by our key results, studies of even longer duration are required. A trend towards the use of continuous combined hormone replacement therapies that include low doses of oestrogensfor example, 1 mg oestradiol or its equivalentand different combinations of hormones means that the safety of such regimens needs to be evaluated continually.

A recent short term study of continuous combined hormone replacement therapy with oestradiol 1 mg and varying doses of norethisterone acetate over one year reported an incidence of endometrial hyperplasia of less than 1%.³⁰ It would be interesting to establish whether the use of continuous combined hormone replacement therapy has a role in the treatment of complex hyperplasia arising in postmenopausal women not taking hormone replacement therapy.